Standard Operating Procedure Colony Counter
STANDARD OPERATING COLONY COUNTER
- Clean the instrument with dry clean cotton cloth.
- Switch ‘ON’ the main power supply.
- Switch ‘ON’ the instrument.
- Connect plug of the Auto Marker Pen to the probe socket provided on instrument.
- Keep the petri dish on the Wolffhuegel glass of instrument.
- Adjust the magnifier lens with the help of adjustable knob as convenient.
- Switch ‘ON’ the illumination
- Press the ‘RESET’ button. The display will show 0000
- Press the ‘AUDIO’ button.
- Select the square of Wolffhuegel grid one by one and mark the colonies with the helpof Auto Markter Pen.
- The marking ensures counting of colonies and audio bepp indicates that the count hasbeen made.
- Marking of all colonies can be observed through magnifier lens and counting should bedone simultaneously.
- Record the total number of cfu count from display in respective annexure.
- Switch ‘OFF’ the illumination
- Switch ‘OFF’ the instrument
- Switch ‘OFF’ the main power supply
- Clean the instrument with clean, cotton cloth.
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